Fig. 1: Establishing an hTLS model.
From: Modelling co-development between the somites and neural tube in human trunk-like structures
a, Schematic of the hTLS protocol with representative brightfield images at each 24-h timepoint. 2D, two-dimensional; embed., embedded. b, Elongation of ZO-1::mEGFP hTLS major axis length (mean ± s.d.) (n = 12–32 per timepoint). c, Projection of immunofluorescence imaging of ZO-1::mEGFP hTLS at 120 h (n = 6). The asterisk indicates somites. d, Projected images of whole-mount, in situ HCR staining of HES7::Achilles hTLS at 120 h (n = 2). The asterisk indicates somites. e, Scanning electron micrograph of a LaminB1::mTagRFP-T hTLS at 120 h (n = 2). Scale bar, 300 μm. f, Confocal section (top) and projection (bottom) of immunofluorescence imaging of a ZO-1::mEGFP hTLS at 120 h. Green arrows highlight clusters of PAX7+ cells (n = 2). The asterisk indicates somites. Scale bars, 100 μm. g, Single-cell transcriptomic UMAP of LaminB1::mTagRFP-T hTLS development (N = 1; n = 15,808 cells). h, The temporal spread of samples collected at 72 h, 96 h and 120 h timepoints. i, Marker gene expression for each identified cluster. j, Projection and subsequent label transfer of high-confidence cell identities from a published human embryo dataset38. Chi, Chiron (CHIR99021); DF, determination front; Mat, Matrigel; NMP, neuromesodermal progenitor; NT, neural tube; PSM, presomitic mesoderm; RAL, retinal; SB43, SB431542; TB, tailbud. Scale bars, 50 μm, unless otherwise stated.
