Extended Data Fig. 8: CellChat Analysis.
From: Modelling co-development between the somites and neural tube in human trunk-like structures
(a) Relative information flow from CellChat6 analysis, identifying unique signalling interactions between the somite cell cluster and neural clusters (combined Neural progenitor populations 1-3 and Neuronal clusters). Dotted line indicates equal information flow = 0.5. (b) Predicted signalling direction of non-canoncial Wnt and RA pathways, as predicted by CellChat. (c) Localised expression of non-canonical Wnt (top; n = 3) and RA (bottom; n = 2) signalling components by HCR in 120 h LaminB1::mTagRFP-T hTLS. Scale bars, 50 μm. (d) Gene expression of signalling ligands and receptors at single cell level across populations. (e) CellChat analysis showing statistically significant interactions between the somite and neuronal populations. Pathway names coloured in purple indicate possible somite-to-neural directional signalling, and names coloured in ochre indicate possible neural-to-somite directional signalling. (f) CellChat-predicted interactions between ligands and receptors, organised by pathway.
