Fig. 3: Characterizing signalling in hTLS models.
From: Modelling co-development between the somites and neural tube in human trunk-like structures
a, Representative stills from live imaging of the HES7-Achilles hTLS at 15-min intervals. White arrows denote the same boundary, showing a posterior shift of the wavefront (n = 33). Scale bar, 100 µm. b, Kymograph of Achilles oscillations from the HES7::Achilles hTLS represented in a. c, Time intervals of HES7 oscillations from peak to peak (mean ± s.d.) (n = 33). d, Schematic of AP signalling gradients across the embryo axial length. e–i, Projected images of HCR staining of ZO-1::mEGFP hTLSs at 120 h for components of the FGF (n = 5) (e), WNT (n = 3) (f) and notch (n = 3) signalling pathways (g); expression of DLL1 within the SOX2-expressing neural tube by confocal imaging (n = 3; scale bars, 100 μm) (h); and RA signalling pathway components along the AP axis (n = 2) (i). j, Localized mediolateral signal of ALDH1A2 in somites and punctate signal of DLL3 through the neural tube of ZO-1::mEGFP hTLS. The dashed box shows the location of the enlarged region (n = 4). Scale bars, 50 μm, unless otherwise stated. The green arrows show DLL1 signal in the neural tube, the asterisks indicate somites and the dashed line outlines hTLS. A, anterior; P, posterior; RA; retinoic acid.
