Fig. 4: DV patterning in hTLS structures.
From: Modelling co-development between the somites and neural tube in human trunk-like structures
a, Schematic diagram of the signalling gradients (left) and subsequent DV patterning of the neural tube (middle), leading to discrete neural populations (right). BMP, bone morphogenetic protein. b, Gene expression of neural populations in hTLS, ordered from top to bottom by expression along the DV axis in embryos. c, Projected images of 144-h LaminB1::mTagRFP-T hTLS treated with increasing concentrations of SAG (SHH pathway activator) and HCR stained for neural markers (n = 5–8 per condition). Dashed line outlines hTLS boundaries. Scale bars, 100 µm. d, Quantification of expression by volume (%) with increased exposure to SAG. Dashed line indicates smoothed trend with 0.95 confidence interval (grey ribbon). Box plots represent mean (centre line) and interquartile range (bounding box), with whiskers representing the largest and smallest value no further than 1.5× the interquartile range and outliers represented as black dots. n.s., non-significant. *P < 0.05; **P < 0.01 obtained by two-sided Wilcoxon test compared with 0 µm SAG control (n = 3–12 per condition). e, Schematic diagrams (left), XY images (middle) and YX optical slices (right) showing HCR gene expression in the 144-h hTLS neural tube with increasing SAG concentrations. Scale bars, 150 µm for SAG 200 nM condition and 100 µm for all others in e.
