Extended Data Fig. 7: Deletion of RASH3D19 sensitizes mutant KRAS expressing PDAC, CRC, and NSCLC cells to RAS inhibitors. | Nature Cell Biology

Extended Data Fig. 7: Deletion of RASH3D19 sensitizes mutant KRAS expressing PDAC, CRC, and NSCLC cells to RAS inhibitors.

From: RASH3D19 mediates RAS activation through a positive feedback loop in KRAS-mutant cancer

Extended Data Fig. 7: Deletion of RASH3D19 sensitizes mutant KRAS expressing PDAC, CRC, and NSCLC cells to RAS inhibitors.

a, Cell viability assays of AsPC1 and MIAPaCa2 cells which were transfected with control sgRNA or sgRNAs targeting RASH3D19 cultured in 3D for 24 h and then treated with different concentrations of MRTX1133 or AMG510 for 3 days. b, RASH3D19 expression levels in SW837, H1972 and MIAPaCa2 cells prepared as in (a). Bar graphs are represented as mean ± SD. P values were calculated using two-sided unpaired Student’s t-tests. ***, P < 0.001. c, Cell viability assay of sgControl and sgRASH3D19 AsPC1 and PANC1 cells which were cultured in 3D for 24 h followed by treatment with different concentrations of RMC4550 for 3 days. All in vitro assays are representative of three independent experiments.

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