Fig. 3: Translation of SG-enriched mRNAs is favoured by G3BPs.
From: The G3BP stress-granule proteins reinforce the integrated stress response translation programme
a, mRNAs enriched and depleted in SGs. Plotted for each mRNA is its abundance in the SG fraction as a function of its abundance in the cytoplasmic fraction. Abundance is reported in reads per million (RPM). Points for SG-enriched and SG-depleted mRNAs, as determined by DESeq2 (log2 FC >1, adjusted P value <0.05), are indicated in red and blue, respectively, with their numbers in parentheses. n indicates the total number of unique mRNAs. b, The relationship between SG enrichment and ISR translation. Plotted are the SG enrichments of mRNAs in each of three ISR translation categories: stress-enhanced/resistant, bulk behaviour and stress-hypersensitive (lines represent the median, notches represent the 95% confidence interval, boxes represent quartiles and whiskers represent 1.5× interquartile ranges (IQRs); ***P = 7 × 10−26, 9 × 10−56 and 6 × 10−26 (from left to right) Welch’s two-sample, two-tailed t-test; n = 10,383 unique mRNAs). c, The relationship between G3BP-dependent translation and SG enrichment. The plots indicate the log2 FC in TE owing to presence of G3BPs in stressed cells for mRNAs in each of three SG-enrichment categories: SG enriched, SG depleted and neither (***P = 2.6 × 10−5, 1.2 × 10−15 and 5.6 × 10−15 (from left to right); otherwise as in b). All stresses were 500 µM sodium arsenite for 1 h.
