Extended Data Fig. 6: Mitochondrial fragmentation is not sufficient to regulate the timing and extent of mitochondrial reduction at OZT.
From: Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance
a, Top: Confocal fluorescence images showing the change in mitochondrial morphology (visualized with NDUV-2::mNG) between the –2 oocyte and zygote in an empty vector control compared to a fzo-1 RNAi-treated animal. Scale bar, 10 µm. Boxed regions are magnified under original images. Bottom: Skeletonized images of the mitochondria shown in middle panels. Scale bar, 2 µm. b, Quantification of mitochondrial network parameters (NDUV-2::mNG) compared between –2 oocytes and zygotes in control and fzo-1 RNAi-treated animals. Data represent mean ± s.d. (n = 30 animals per condition) from three biological replicates. P values using two-tailed Student’s t-tests and two-tailed Mann–Whitney tests, depending on normality of distribution. c, Quantification of MOZT in control compared to fzo-1 RNAi-treated animals. Data represent ratio of total NDUV-2::mNG fluorescence intensity in the zygote to that in the –2 oocyte for each animal. The data represent the mean ± s.d. (n = 30 animals each condition) from three biological replicates. P value using a two-tailed Student’s t-test. d, Quantification of mean volume (µm3) of individual fragmented mitochondrion, autophagosome, and lysosome in the –1 oocyte. Data represent mean ± s.d. (n = 30 animals per strain) from three biological replicates. P values using a Kruskal–Wallis test followed by a Dunn’s multiple comparisons test.
