Extended Data Fig. 10: Reproductive decline and embryonic lethality in fndc-1 (UV) lines are strictly maternal effects.
From: Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance
a, A schematic of the experimental design to rescue sperm health in fndc-1 UV lines. Green animals denote untreated worms, and magenta animals denote worms with mtDNA damage. b, Quantification of reproductive fitness (brood size) of self-fertilizing hermaphrodites from fndc-1 UV-bottlenecked lines (fourth generation, G4) compared to those crossed with untreated wild-type males. Data represent mean ± s.d. (n = 11 animals [selfed] and 10 animals [crossed]) from three biological replicates. P values using a two-tailed Student’s t-test. c, Verification of successful crossing between wild-type males and hermaphrodites from fndc-1 UV-bottlenecked lines (G4). Merged confocal images showing F1 progeny with paternally inherited plasma membrane signal (mCherry::PH, magenta; DIC, grey). Magenta signal in progeny confirms successful cross-fertilization by wild-type males. Scale bar, 10 µm. d, Schematic of experimental design to rescue embryonic expression of FUNDC1 in fndc-1 UV-bottlenecked lines (G4). Green denotes the untreated condition, and magenta denotes UV-treated animal. e, Embryonic lethality among F1 progeny of parents from the fndc-1 UV-bottlenecked lines (G4) compared to F1 progeny of parents (from the same lines) injected with FNDC-1 rescuing embryo expression plasmid [pie-1p::mRb3::fndc-1::tbb-2 3′UTR]. Data represent mean ± s.d. (n = 10 animals per condition) from three biological replicates. P values using a two-tailed Mann–Whitney test. f, Merged confocal fluorescence images of mRb3::FNDC-1 (FNDC-1, magenta; DIC, grey) in oocytes of a fndc-1 UV-bottlenecked (G4) parent injected with embryo rescue plasmid and the resulting F1 embryo. Magenta signal in the F1 embryo, but not the parental oocytes confirm embryo-specific rescue of mRb3::FNDC-1 expression. Scale bar, 10 µm.
