Extended Data Fig. 5: Validation of hGCase/eGFP expression in dopaminergic neurons.
From: Glucosylceramide-induced ectosomes propagate pathogenic α-synuclein in Parkinson’s disease
a-b, Cytotoxicity assessment of the GCase modulator 758 on GBA1-N370S, LRRK2-G2019S and corresponding isogenic control neurons by MTT. n = 4 independent experiments; 3 technical replicates/experiment; mean ± SEM; One-way ANOVA with Dunn’s multiple comparison. c-h, Western blotting of GBA1-N370S, LRRK2-G2019S and corresponding isogenic control neurons expressing hGCase/eGFP or eGFP and immunoblotted for hGCase, GFP and β-actin. Expression of hGCase and eGFP normalized against β-actin. n = 4 independent experiments, mean ± SEM. i-j, Percentage of eGFP positive dopaminergic neurons in GBA1-N370S, LRRK2-G2019S and corresponding isogenic controls. n = 4 independent experiments; 4 tile scans /2 coverslip/experiment; mean ± SEM. k-l, Cytotoxicity assessment of hGCase/eGFP expression on GBA1-N370S, LRRK2-G2019S and corresponding isogenic control neurons by MTT. n = 4 independent experiments; 3 technical replicates/experiment; mean ± SEM; One-way ANOVA with Dunn’s multiple comparison. Graphs are depicted as superplots where biological replicates are shown in large shapes, and where applicable technical replicates are shown as small shapes.
