Extended Data Fig. 7: Peptide CUE3 enhanced pyroptosis. | Nature Cell Biology

Extended Data Fig. 7: Peptide CUE3 enhanced pyroptosis.

From: TOLLIP targets GSDME-NT-carrying endocytic vesicles for autophagy to regulate pyroptosis and chemotherapy efficacy

Extended Data Fig. 7: Peptide CUE3 enhanced pyroptosis.The alternative text for this image may have been generated using AI.

(a) Immunofluorescence images and the localization ratio (n = 30 cells) of LC3 with TOLLIP in cells treated with Dox and Peptide CUE3. Scale bar: 10 μm. (b) Secreted IL-6 and HMGB1 levels in HK1 GSDME-FL-HA cells treated with raptinal and Peptide CUE3. (c-d) Immunoblots (c), HMGB1 release, 7-AAD staining, LDH activity and IL-6 secretion (d) analysis of HK1 cells pretreated with decitabine, under cisplatin with or without Peptide CUE3 exposure. (e) Flow cytometry analysis of HER2 surface expression in HONE1, HK1 and MDA-MB-361 cells. (f-h) Immunoblots (f), HMGB1 release (g), 7-AAD staining, LDH activity and IL-6 secretion (h) in HK1 cells pretreated with decitabine, exposed to CAR-T cells, with or without Peptide CUE3. Data are shown as Mean ± SD. Data are representative of at least three independent experiments (a-h). Two-tailed unpaired Student’s t test (a, b, d, h) was used for analysis.

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