Extended Data Fig. 8: Crosstalk of autophagy and GSDME-NT mediated pyroptosis. | Nature Cell Biology

Extended Data Fig. 8: Crosstalk of autophagy and GSDME-NT mediated pyroptosis.

From: TOLLIP targets GSDME-NT-carrying endocytic vesicles for autophagy to regulate pyroptosis and chemotherapy efficacy

Extended Data Fig. 8: Crosstalk of autophagy and GSDME-NT mediated pyroptosis.The alternative text for this image may have been generated using AI.

(a) Representative immunofluorescence images of mRFP-GFP-LC3B-transfected cells treated with Dox. Scale bar: 10 μm. (b) Representative TEM images showing the diaminobenzidine staining pattern in APEX2-LC3B-transfected cells. (c) Schematic of the processing assay using Halo-LC3 as an autophagy reporter. Halo-LC3 is completely degraded in autolysosomes. Upon labelling with a TMR-conjugated ligand, LC3 is degraded in autolysosomes, releasing ligand-bound Halo fragments (Halo-ligand) that are resistant to further degradation. (d) Immunoblots of HK1 GSDME-NT-T6E-HATet-On and GSDME-NT-F2A + K40A-HATet-On cells treated with Dox for 6 h, with or without CQ. (e) Immunoblots showing the expression of AMPKα in AMPK DKO cells. (f) Immunoblots of HK1 GSDME-NT-HATet-On cells treated with Dox for 6 h, with or without MK-8722. (g) Immunoblots of lysates of HONE1 and HK1 cells overexpressing GSDME-NT-HATet-On treated with Dox (left) or cells overexpressing GSDME-FL treated with raptinal (right). Data are representative of at least three independent experiments (a, b, d-g).

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