Extended Data Fig. 8: Spatial and temporal variation of shotgun sequenced air and swab eDNA.

a) Area-proportional Venn diagrams of non-deuterostome eukaryotic genus hit overlaps between co-sampled (same time at the same site, Supplementary Table 1) Florida forest swab January 2024 eDNA samples. Short-read shotgun sequencing, only genera with over 20 supporting reads are shown [NT r]. Left: wood plank, palm leaf and metal post swabs. Right: Windowpane, palm leaf and metal post swabs. b) Temporal comparison, area-proportional Venn diagram of non-deuterostome eukaryotic genus hit overlaps between air samples collected at the same site, at different times, or different durations, Florida forest air eDNA, i) 1 week Nov. 2023, ii) 3 weeks Nov. 2023 or iii) 1 week April 2024 (Supplementary Table 1). Sample name abbreviation: FOR = forest, 1wk = 1 week, 3wk = 3 weeks. Short-read shotgun sequencing, only genera with over 20 supporting reads are shown [NT r]. c) All domains of life (excl. deuterostomes) top genera heatmap of the same three air eDNA samples shown in b) above. Short-read shotgun sequencing, heatmap displays the 30 highest read genera from each sample. d) Spatial comparison, area-proportional Venn diagram of non-deuterostome eukaryotic genus hit overlaps between 1 week air samples taken for the same time and duration, two sites 3.2 km apart (Florida forest [FOR] & Whitney Lab [WL], Supplementary Table 1).