Extended Data Fig. 5: In vitro toxicity assessment of Neo-4 compared to IL-4.
From: Design of cell-type-specific hyperstable IL-4 mimetics via modular de novo scaffolds
a-b, Human (a) or mouse (b) CD4+ T cells were subjected to Th2 polarization conditions through treatment with titrated amounts of IL-4 or Neo-4 for 1, 3, and 5 days. Live cell count, viability, and normalized reactive oxygen species staining are shown. Data represent mean ± SD (n = 2). c-d, LDH activity from supernatants of human CD4+ T cells (c) and human monocytes (d) polarized with titrated amounts of IL-4 or Neo-4 for 1, 3, and 5 days, as quantified by colorimetric assay. Data represent mean ± SD (n = 2).
