Extended Data Fig. 9: Thermal stability characterization of IL-4 mimetics.

a, Thermal denaturation curves for hNeo-4 and Neo-2. b, Circular dichroism (CD) spectra of hNeo-4 at pre-heating (25 °C), melting (95 °C), and post-heating (25 °C) stages. c, Thermal denaturation curve for mNeo-4. d, CD spectra of mNeo-4 pre-heating (25 °C), melting (95 °C), and post-heating (25 °C) stages. e, Schematic of the manufacturing and characterization of a PCL-based 3D-printed scaffold incorporating hNeo-4. f, STAT6 phosphorylation induced by hIL-4, hNeo-4, and lyophilized hNeo-4 in Ramos B cells. Data represent mean ± SD (n = 2) g, Release profile of hNeo-4 (percentage mass) from 3D-printed scaffolds, as measured by fluorescent protein quantification assay. Data represent mean ± SD (n = 3 biologically independent samples). h, Release profile of hNeo-4 (mass) from 3D-printed scaffolds, as measured by fluorescent protein quantification assay. Data represent mean ± SD (n = 3 biologically independent samples). i, Quantification of hNeo-4 mass released from 3D-printed scaffold over 16 h incubation at 4 °C, as measured by fluorescent protein quantification assay. Data represent mean ± SD (n = 9 biologically independent samples). j, Percentage mass of hNeo-4 released from 3D-printed scaffold over 16 h incubation at 4 °C, as calculated based on the measurements from fluorescent protein quantification assay shown in i. Data represent mean ± SD (n = 9). k, STAT6 signaling activation induced by hNeo-4 from 3D-printed scaffolds on Ramos B cells. Signaling stimulated by either hNeo-4 released from 3D-printed scaffolds (M) or hNeo-4 on the surface of 3D-printed scaffolds (S) is shown. Signaling of unmodified PCL scaffolds is shown for reference. Data represent mean ± SD (n = 3 biologically independent samples).