Extended Data Fig. 6: The eCAGr RNA impairs the protein translation elongation.
From: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis
a. Representative gel images and quantifications of puromycin-labeled proteins in WT and HD mouse striatal cells in SUnSET assays. b. The plot of SunRiSE assay signals (detected by high-content imaging) showing a decrease of translation elongation in WT versus HD mouse striatal cells. c. Representative polysome profiles and quantifications of WT/HD mouse striatal cells or the transfected WT mouse striatal cells expressing 72 × CAG RNA, before harringtonine treatment versus after 3 min harringtonine treatment. The polysome-to-monosome (P/M) ratio before and after treatment was calculated and plotted to represent the run-off rate. Mean ± s.e.m.; two-tailed unpaired t test (a, c) or two-way ANOVA (b). n= # of biologically independent samples (a–c). ****: p < 0.0001.
