Extended Data Fig. 8: eEF2 showed weaker association with the ribosomes in eCAGr RNA expressing cells.
From: Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis
a. Representative western-blots and quantifications with the corresponding polysome profiles showing the distribution of eEF2 and eEF1A in the indicated polysome fractions of WT and HD mouse striatal cells with the indicated transfection and/or treatment. The presence of eEF2 in the ribosome fractions was reduced in HD cells. b. Representative fluorescence images (from 8) of endogenous eEF1A, eEF2 and Rps6 (a ribosome marker) in WT or HD mouse striatal cells treated with the lysosome inhibitor NH4Cl or 8 × CTG. Colocalization patterns between eEF1A/eEF2 and Rps6 at the yellow lines are visualized. The eEF2-Rps6 colocalization was drastically impaired. Mean ± s.e.m.; one-way ANOVA with multiple comparisons (a). n= # of independent experiments (a). Scale bars, 5 μm.
