Extended Data Fig. 4: Genetic Boolean logic enables concentration-dependent transcriptional responses in S. oneidensis expressing sfgfp.
From: Transcriptional regulation of living materials via extracellular electron transfer
Relative Expression Units (REU) measured as a function of combinatorial inducer concentration show characteristic transcriptional regulation that follows expected truth tables for multiple genetic Boolean architectures expressing sfgfp (OR, NOR, NAND) or eyfp (AND). S. oneidensis MR-1 harboring each plasmid was grown overnight in aerobically prepared 96-well plates that were then sealed to emulate dynamic cross-linking conditions. Fluorescence was OD600-normalized and referenced to a constitutive fluorescence plasmid to obtain REU. The expected truth tables are shown for maximum and minimum induction conditions (0, ‘OFF’; 1, ‘ON’). Data shown are mean of n = 3 biological replicates.
