Extended Data Fig. 5: Genetic Boolean logic enables synthetic material computation during stationary phase cross-linking.
From: Transcriptional regulation of living materials via extracellular electron transfer
a, Storage moduli for networks cross-linked using S. oneidensis strains harboring transcriptional Boolean logic circuits controlling mtrC expression under stationary phase conditions. b, The logical architectures span i, OR, ii, NOR, iii, NAND, and iv, AND. In all cases, storage modulus was measured 2 h after inoculation. The expected truth tables are represented below each circuit (0, ‘OFF’; 1, ‘ON’). Each network appropriately responds to combinatorial inputs by increasing/decreasing storage modulus in response to mtrC activation/deactivation. Each plasmid architecture is shown as a cartoon above the corresponding response function, with representations as in Fig. 3. Statistics performed are the results of a general linear hypothesis test (a contrast test) between the ‘OFF’ and ‘ON’ states. Stars reference p value (* p < 0.05, ** p < 0.01, *** p < 0.001), R script is provided in Data Availability, and data shown are mean ± SEM of n = 3 biological replicates.
