Extended Data Fig. 1: S. oneidensis retains viability after cross-linking and remains metabolically active within hydrogels for at least one week.
From: Transcriptional regulation of living materials via extracellular electron transfer
a, BacLight live/dead staining of S. oneidensis MR-1 after cross-linking, swelling overnight in 1x PBS, and mechanical characterization by rheology. Cells are predominantly alive (green fluorescence) as opposed to dead (red fluorescence). b–c, Overlaid fluorescence and bright-field microscopy of S. oneidensis MR-1 + sfgfp (left) encased in gels b, one day or c, one week after cross-linking and swelling, and (right) 24 h after inoculating with 1 mM IPTG to induce fluorescence. Images are representative of n = 3 biological replicates.
