Extended Data Fig. 2: Dynamic cross-linking couples sensing, computation, and actuation in a synthetic material.
From: Transcriptional regulation of living materials via extracellular electron transfer
a–d, Cross-linking can be transcriptionally regulated using the LacI-PtacsymO regulator-promoter pair controlling mtrA (a) (c) or cymA (b), (d) expression under a-b, stationary phase or c-d, dynamic conditions. Data are fit to an activating gene expression model and right axis is normalized to storage modulus of gels formed using wild-type S. oneidensis harboring an empty vector. Dashed lines represent gel mechanics using corresponding knockout strains harboring an empty vector; if no line is shown, gels did not form. Data shown are mean ± SEM of n = 3 biological replicates. e-f, The difference between the induced and uninduced storage modulus can be controlled via both initial inoculation density and reaction time for LacI-PtacsymO regulator-promoter pair controlling mtrA (e) or cymA (f) (heatmap data represents n = 1).
