Extended Data Fig. 8: Cryo-EM data processing for RNF1681–113/UbcH5c–Ub/NCP complex achieved by ACC strategy. | Nature Chemical Biology

Extended Data Fig. 8: Cryo-EM data processing for RNF1681–113/UbcH5c–Ub/NCP complex achieved by ACC strategy.

From: Mechanism of nucleosomal H2A K13/15 monoubiquitination and adjacent dual monoubiquitination by RNF168

Extended Data Fig. 8: Cryo-EM data processing for RNF1681–113/UbcH5c–Ub/NCP complex achieved by ACC strategy.

a, A representative micrograph. The micrographs collected in this dataset show similar particle density and dispersion to the representative micrograph. b, Representative 2D classifications. c, Data processing flowchart. d, Cryo-EM density map at an overall resolution of 6.87 Å representing the closed E2~Ub conformation. e, Local resolution analysis of the 3.39 Å cryo-EM density map. f, Gold standard Fourier shell correlation (FSC) curves showing the overall resolution of 3.39 Å and 3.27 Å for the final density map of RNF1681–113/UbcH5c–Ub/NCP and RNF1681–113/UbcH5c/NCP conformation, respectively.

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