Extended Data Fig. 2: Cryo-EM data processing for RNF1681–113/UbcH5c–Ub/NCP complex achieved by SICC strategy. | Nature Chemical Biology

Extended Data Fig. 2: Cryo-EM data processing for RNF1681–113/UbcH5c–Ub/NCP complex achieved by SICC strategy.

From: Mechanism of nucleosomal H2A K13/15 monoubiquitination and adjacent dual monoubiquitination by RNF168

Extended Data Fig. 2: Cryo-EM data processing for RNF1681–113/UbcH5c–Ub/NCP complex achieved by SICC strategy.

a, A representative micrograph. The micrographs collected in this dataset show similar particle density and dispersion to the representative micrograph. b, CTF estimation of the micrograph shown in a. c, Representative 2D classifications. d, Data processing flowcharts. e, Euler angle distributions of the final particles for cryo-EM reconstruction. f, Local resolution colored cryo-EM density map. g, Gold standard Fourier shell correlation (FSC) curves showing the overall resolution of 3.27 Å and 3.62 Å for the final density map of RNF168/UbcH5c–Ub/NCP, and RNF168/UbcH5c, respectively.

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