Extended Data Fig. 2: Concentration-response curves for 17 of the new docking-derived AmpC inhibitors.
From: The impact of library size and scale of testing on virtual screening
Nitrocefin was kept at a constant concentration of 100 μM (for positive control ZINC549719643, new inhibitors Z6615018018, Z6615017509, Z6615022372, Z6615017782, Z6615020275, Z6615019214 and Z6615014610) or 50 μM (for positive control ZINC339304163, new inhibitors Z2275216423, Z6615017736, Z2940316600, Z2940315182, Z6615019960, Z2940322517, Z6615017422, Z6615015266, Z6615016774 and Z6615155291). The estimated Ki is calculated based on the Kd of nitrocefin (180 μM) calculated from a Lineweaver-Burk analysis. The previously reported Ki for ZINC549719643 is 77 nM1 and for ZINC339304163 is 1.25 μM1. Data represent mean ± s.d.s from three biological replicates.
