Extended Data Fig. 1: Temperature effects on phase transition and aggregation of HSF1.
From: The molecular mechanism of temperature-dependent phase separation of heat shock factor 1
a. The correlation between species body temperature and the middle point temperature of the phase transition curve. b. The box-plots of droplet sizes in Fig. 1e. n = 79 for WT-25 °C, n = 100 for WT-37 °C, n = 71 for M1-5 °C, n = 94 for M1-25 °C and n = 85 for M1-37 °C across 3 independent experiments. ND, not determined. c. The peak with a retention time of 10 ml was the LZ1-3 oligomers by SEC-MALS. The UV absorbance values were in red, and the light scattering values were in gray. The molar mass values (g/mol) were in blue. d. DSS cross-linked lz1-3 at different temperatures were detected by SDS‒PAGE, which is representative of 3 independent experiments. e. The box-plots of droplet sizes in Fig. 1f. n = 86 for WT-25 °C, n = 190 for WT-37 °C, n = 153 for M1-5 °C, n = 153 for M1-25 °C and n = 151 for M1-37 °C across 3 independent experiments. ND, not determined. In the box plots, data are presented as mean values, box plots: 25th to 75th percentiles, median line, 1.5× interquartile as whiskers and points.
