Extended Data Fig. 7: Supporting experimental results for intracellular ROMP mediated by Ru–HS–ArM in compartmentalized HS–E. coli.
From: Artificial metalloenzyme assembly in cellular compartments for enhanced catalysis
a, Chemical scheme of the ROMP of NB-Ant monomer. b, Fluorescence emission spectra of NB-Ant (left) and poly(NB-Ant) (right) in different DMF/water mixtures. The results clearly indicated the aggregation-induced emission (AIE) property of the polymerized anthracene units. Concentration: 0.1 mg/mL, Ex = 370 nm. c, 1H NMR spectra overlay of the monomer (NB-Ant) and the product polymer (poly(NB-Ant)) involved in the intracellular ROMP in HS–E. coli. The characteristic backbone alkene peak (5.4–5.8 ppm) and the broadening of the peaks clearly indicated the successful formation of polynorbornene. d, Comparison of the molar mass distribution of the poly(NB-Ant) obtained from living ROMP (performed in dichloromethane) and in cellulo ROMP mediated by Ru–HS–ArM in artificial compartments.
