Extended Data Fig. 8: Phylogenetic analysis of KS-AT linkers from aminopolyol PKSs according to the upstream domain organization.
From: Plug-and-play engineering of modular polyketide synthases
a. Phylogenetic tree of the KS-AT linkers. The tree was constructed by FastTree2.1.1 with branch support values, performed on Geneious Prime. Labels indicate sequence source and location in a PKS pathway. For example, ecoM2c indicates the KS-AT linker in the ECO-02301 pathway (eco) at the second PKS (2), third module (c) based on traditional module definition shown in panel b. eco: ECO-02301; med: mediomycin; tfb: tetrafibricin; nmd: neomediomycin; lin: linearmycin; cle: clethramycin; S.mel: Streptomyces melanospoorfaciens PKS; S.nbrc: Streptomyces. sp. NBRC 109436 PKS; S.RTd: Streptomyces. sp. RTd22 PKS; S.ven: S. venezuelae PKS S.mash: S. mashuensis PKS; K.med: Kitasatospora mediocidica PKS. Accession numbers for the PKSs are provided in Supplementary Information. b. The module duplication observed in PKS1s of aminopolyols, based on Zhang et al. 201714. Sequences with high similarity are colored with yellow (KR-containing module) and dark yellow (DH-KR-containing module) to illustrate module duplication. Types of KS-AT linkers from the PKS1s of each pathway are shown in circle, triangle, and box as explained in panel c. The clear separate clades were observed in the tree depending on the types of its upstream domains, suggesting that KS-AT linkers also comigrate with its upstream sequences during module duplication.
