Extended Data Fig. 5: Biosynthetic schemes of PKSs with β-processing domains.
From: Plug-and-play engineering of modular polyketide synthases
a. Proposed product formation mechanism by Rak-Vem-4 pathway. 2a can be produced by β-processing domain (DH-ER-KR) skipping; 2c can be produced by β-processing domain (DH-ER-KR) skipping and an iterative elongation at VemH; 2d was produced by fully functional β-processing domains and an iterative elongation of VemH. b. Proposed product formation mechanism by DEBS-Vem-4 pathway. 3a was produced by KR skipping; 3c was produced by fully functional domains. Iterative elongation during in vitro reaction of modular PKS has been documented previously66.
