Extended Data Fig. 5: CLMS analysis of the Nb23-bi-DHEA-ADGRG2-Gs complex by two cross-linkers (DOPA2 or DSS).
From: Development of an allosteric adhesion GPCR nanobody with therapeutic potential
a. Annotated MS/MS spectrum of the DOPA2-linked intermolecular peptide pair. b. Annotated MS/MS spectrum of the DSS-linked intermolecular peptide pair. The same two cross-links identified by both DSS and DOPA2 were not shown. c-d. Table of the cross-linked pairs identified between ADGRG2 and Nb23-bi following treatment with DOPA2 (c) or DSS (d). Numbers indicate positions of lysines cross-linked on ADGRG2 or Nb23-bi; Cross-linked residue in peptide sequence is highlighted in bold.
