Extended Data Fig. 7: Reversibility of the reverse reaction as demonstrated by solvent exchange in D2O with SSpBesB.
From: Terminal alkyne formation by a pyridoxal phosphate-dependent enzyme
a) All four non-acidic protons of 3 exchanged with D2O solvent when SSpBesB is present (Ammonium Bicarbonate Buffer, pH = 7.8). b) The masses detected by LC/MS-QTOF for each exchange of 3 in D2O.
