Extended Data Fig. 2: Purification and activity data for SAcBesB.
From: Terminal alkyne formation by a pyridoxal phosphate-dependent enzyme
a) Propargylglycine biosynthetic gene cluster in S. achromogenes. b) SDS-PAGE gel of purified SAcBesB. c) Gel filtration data for SAcBesB. Protein standards for alcohol dehydrogenase, bovine serum albumin, and carbonic anhydrase indicated. d) Compound 3 production by SAcBesB using 1 as a substrate. e) Compound 3 production by SAcBesB using 2 as a substrate. Reactions repeated in triplicate with representative results shown.
