Extended Data Fig. 6: GDP or GTP promotes CDC42 binding preference to RhoGDI or KLHL23.
From: KLHL23 and RhoGDI coordinate CDC42 inactivation ensuring membrane homeostasis
a,b, Ribbon representation of the shift of switch I and II of CDC42 by GDP or GTP binding to interact with RhoGDI or KLHL23. White-dashed circles: intramolecular H bonds formed between the third phosphate of GTP with the switch I and II of CDC42. Red dashed circles: inter-molecular H bonds formed between the third phosphate of GTP with RhoGDI (a) or KLHL23 (b). c, Protein-protein interaction by using immunoprecipitation. HEK 293 T cells were co-transfected with KLHL23 (upper panel) or RhoGDI (lower panel) and myc-tagged vector and CDC42 variants. Wt: wild-type CDC42. Immunoprecipitation was performed by using anti-myc antibodies.
