Extended Data Fig. 7: Colocalization of KLHL23 and RhoGDI in the plasma membrane.
From: KLHL23 and RhoGDI coordinate CDC42 inactivation ensuring membrane homeostasis
a, GFP-CDC42, GFP-KLHL23, and GFP-RhoGDI co-localize at the leading edge of the plasma membrane in migrating cells. Arrowheads indicate the plasma membrane edge at the leading front of migrating cells, as shown in the differential interference contrast (DIC) image (left panel). K23: KLHL23; C42: CDC42; GDI: RhoGDI. Scale bar = 10 μm. b, Time-lapse imaging for GFP-KLHL23 colocalized with the plasma membrane during filopodia formation of a HeLa cell 15 min after being reseeded on the fibronectin-coated plates. Left panel: A representative merged image shows colocalization of KLHL23 with PM at the plasma membrane and filopodia.
