Extended Data Fig. 3: RhoGDI facilitates KLHL23 selectively targeting active CDC42·GTP.
From: KLHL23 and RhoGDI coordinate CDC42 inactivation ensuring membrane homeostasis
a, In vitro polyubiquitylation assays reveal the effects of RhoGDI on KLHL23-mediated CDC42 polyubiquitylation. b, Immunoblots assessing the impact of RhoGDI on the levels of CDC42 variants in HEK293 cells with or without KLHL23 depletion. WT, Q61L, and T17N represent wild-type, constitutively active, and inactive CDC42 variants, respectively. c, The silencing of endogenous RhoGDI sensitizes T17N-CDC42 variant to KLHL23-mediated degradation.
