Extended Data Fig. 7: Nuclear speckle morphology under compound treatments.
a, U2OS-2XALFA-SON cells were stained with HaloTag TMR ligand and treated with 100 nM BafA1, 20 μM CCCP, 4 mM DTT, 100 μM H2O2, 1 μM TG or 2.5 μg/ml TM for 2 h. Images were acquired using an FV3000 confocal laser scanning microscope. b,c, Quantitative analysis of the roundness (b) and the Feret’s diameters (c) of nuclear speckles in control and treatment groups. Speckle counts: Control, n = 657; BafA1, n = 479; CCCP, n = 686; DDT, n = 475; H2O2, n = 948; Oligomycin A, n = 559; Rapamycin, n = 682; TG, n = 436; TM, n = 524. Data are expressed as the mean ± s.e.m. Significance was calculated by two-tailed Student’s t-test. CCCP: Carbonyl cyanide 3-chlorophenylhydrazone, DTT: dithiothreitol, H2O2: hydrogen peroxide, TG: thapsigargin, TM: tunicamycin.
