Fig. 3: Cryogenic soft X-ray microscopy (cryo-SXM) and XANES reveal amorphous guanine within intracellular organelles.
From: pH variations enable guanine crystal formation within iridosomes
a, Schematic illustration of the experimental design and data collection process using (cryo-SXM), which enables correlated cryo-SXT and XANES. b,e, Cryo-SXM images of single cell from isolated zebrafish larvae iridophores at 1 mpf (b) and 72 hpf (e). Single-cell boundaries are labeled with cyan dashed line. In total, 25 different cells isolated from dozens of larvae were imaged and analyzed. c,f, Two-dimensional volume slices from a reconstructed cryo-SXT of a region within the 1 mpf cell (c) and 72 hpf cell (f), showing multiple crystals and organelles. d,g, XANES 2D heat maps showing the distribution of nitrogen levels within a 1 mpf cell (d) and 72 hpf cell (g).
