Fig. 1: Design concept for synthetic Notch agonists.
From: Engineering synthetic agonists for targeted activation of Notch signaling
a, Flow cytometry histogram overlay of Notch1 reporter cells stimulated by soluble or plated (nonspecifically adsorbed) DeltaMAX. The cartoon depicts the site-specifically biotinylated DeltaMAX (N–EGF5) construct. b, Histogram overlay of Notch1 reporter cells stimulated with soluble or plated DeltaMAX–Fc protein. c, Histogram overlay of Notch1 reporter cells stimulated with plated or soluble DeltaMAX–SA tetramers. d, Cartoon schematic depicting the ECDs of Notch1 and DLL4 interacting during canonical Notch activation. The NRR and ligand-binding domains (LBDs) of Notch1 (EGF domains 8–12) are shaded. e, Schematic of a generalized SNAG construct alongside a cartoon depicting SNAG-mediated Notch activation. f, Representation of the modular design of SNAGs. g–i, Top: schematic highlighting targets of SNAGs separated by canonical Notch ligands (g), tumor biomarkers (h) and B cell biomarker (i). Bottom: the corresponding endocytic mechanism.
