We developed the prime editing tool PIE, which produces precise inversions of large genomic segments and chromosomal structural variations in mammalian cells. PIEv3b achieves high inversion efficiency and outperforms nuclease- and integrase-based methods, enabling chromosome reconfiguration from metacentric to telocentric forms.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$32.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
References
Pacesa, M., Pelea, O. & Jinek, M. Past, present, and future of CRISPR genome editing technologies. Cell 187, 1076–1100 (2024). A review that retraces and projects the development of CRISPR genome editing technologies, from their origins to the latest breakthroughs and future directions.
Lee, J., Kweon, J. & Kim, Y. Emerging trends in prime editing for precision genome editing. Exp. Mol. Med. 57, 1381–1391 (2025). A review that summarizes recent advances in prime editing and related technologies, highlighting emerging trends and applications in precision genome engineering.
Pagnamenta, A. T. et al. The impact of inversions across 33,924 families with rare disease from a national genome sequencing project. Am. J. Hum. Genet. 111, 1140–1164 (2024). This paper reports a systematic analysis of inversion variants and their impact on disease based on whole-genome sequencing of 33,924 rare disease families.
Kweon, J. et al. Targeted genomic translocations and inversions generated using a paired prime editing strategy. Mol. Ther. 31, 249–259 (2023). This paper reports a prime editor nuclease-based method for generating targeted genomic translocations and inversions.
Anzalone, A. V. et al. Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nat. Biotechnol. 40, 731–740 (2022). This paper reports the development of the twin prime editing system, which enables programmable deletion, replacement, integration and inversion of large DNA sequences.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zhang, A. et al. Efficient and precise inversion of genomic DNA from large to chromosomal scale. Nat. Chem. Biol. https://doi.org/10.1038/s41589-025-02033-9 (2025).
A.Z. and H.Y. used ChatGPT to help prepare their contribution to this Research Briefing.
Rights and permissions
About this article
Cite this article
Prime editing-based tool enables precise genomic inversions at kilobase to chromosomal scale. Nat Chem Biol 22, 167–168 (2026). https://doi.org/10.1038/s41589-025-02073-1
Published:
Version of record:
Issue date:
DOI: https://doi.org/10.1038/s41589-025-02073-1
