Extended Data Fig. 4: Representative whole-cell current of CBTA/CaTMD binding site mutants.
From: A single allosteric site merges activation, modulation and inhibition in TRPM5
a-d, Representative traces of whole-cell currents in CaTMD mutant (L764A, n = 4) and controls (non-transfected tsA201 cells, TRPM5-WT), measured either without CBTA (a and c) or with 100 nM CBTA (b and d). The pipette solution contains 100 nM free calcium in (a and b) and 300 nM free calcium in (c and d). Bar graphs show mean currents measured at +100 mV and −100 mV for each group (n = 4-5); each dot corresponds to an independent cell measurement. e-f, Representative traces of whole-cell currents in CaTMD mutants (Q771A, D797A, D797N) and controls (non-transfected tsA201 cells, TRPM5-WT), measured either without CBTA (e) or with 100 nM CBTA (f). The pipette solution contains 100 nM free calcium. Bar graphs show currents measured at +100 mV and −100 mV for each group (n = 4); each dot corresponds to an independent cell measurement. g-h, Representative traces of whole-cell currents in CaTMD mutants Q771A and D797N with 300 nM free calcium, measured either without CBTA (g) or with 1 μM CBTA (h).
