Extended Data Fig. 9: Single-particle cryo-EM data processing of VcLmuACB-59bp DNA complex and VcLmuA-tetramer complex.
From: Structural insights into type-I and type-II Lamassu antiphage systems
a, Representative cryo-EM micrograph of VcLmuACB-59bp DNA. b, Workflow of cryo-EM data processing of VcLmuACB-59bp DNA. c, Reference-free 2D class average of VcLmuACB-DNA. d, Reference-free 2D class average of VcLmuA-tetramer. e, Gold standard FSC plots for the 3D reconstructions of the whole map of VcLmuACB-DNA, calculated in cryoSPARC. f, Gold standard FSC plots for the 3D reconstructions of the whole map of VcLmuA-tetramer, calculated in cryoSPARC. g, Euler angle distribution of the particle image of VcLmuACB-DNA. h, Euler angle distribution of the particle image of VcLmuA-tetramer. i, The final map resolution is color coded for different regions of VcLmuACB-DNA. j, The final map resolution is color coded for different regions of VcLmuA-tetramer.
