Extended Data Fig. 6: PDZD8, ORP1L and TEX2 do not exhibit dynamic recruitment to PA-enriched membranes.
From: Membrane editing with proximity labeling reveals regulators of lipid homeostasis
a, b, Confocal images of cells co-expressing PDZD8-EGFP (a) or EGFP-ORP1L (b) and superPLD or deadPLD targeted to lysosomes (Lyso). Images acquired 0, 30, and 60 min following recruitment of superPLD or deadPLD induced by intermittent blue light illumination (470 nm, 5 s per 1 min), representative of three biological replicates, are shown. Scale bars, 10 μm. c, Confocal images of cells expressing TEX2-EGFP, with co-expression of superPLD or deadPLD targeted to lysosomes (Lyso). Images acquired 60 min following superPLD/deadPLD recruitment, representative of three biological replicates, are shown. Scale bars, 5 μm. d, Zoomed-in images of (c) for the areas marked with the dashed rectangles. Scale bars, 2 μm.
