Extended Data Fig. 10: Membrane editors can produce a variety of unnatural phospholipids in cells.
From: Membrane editing with proximity labeling reveals regulators of lipid homeostasis
a–d, LC–MS quantification of phosphatidyl alcohol lipid species extracted from HEK 293T cells expressing PM-targeted iLID-LOVPLD treated with butanol (a), ethanol (b), butynol (c), or azidopropanol (d), demonstrating that LOVPLD can mediate in situ production of phospholipids with customizable alcohols in light-dependent manner. Total cellular lipids were extracted and analyzed after a 15 min incubation with intermittent blue light illumination (470 nm, 5 s per 1 min). Black horizontal bars indicate means and vertical error bars indicate standard deviations. e–h, Similar quantification of PA species extracted from cells treated with butanol (e), ethanol (f), butynol (g), or azidopropanol (h), showing the lack of noticeable increase in PA levels.
