Extended Data Fig. 3: Mitochondrial dysmorphology in cells with hyperactivated PA production.
From: Membrane editing with proximity labeling reveals regulators of lipid homeostasis
a, Confocal microscopy of HEK 293T cells expressing plasma membrane or lysosome-targeted superPLD or deadPLD. Mitochondria were stained with MitoTracker Green. b, c, Quantification (b) of mitochondrial morphology (Normal, Mix, or Round) in cells expressing LOVPLD anchored on endoplasmic reticulum (ER), lysosome (Lyso), or plasma membrane (PM) over the indicated time periods (0–30 min for ER and 0–180 min for Lyso and PM). 50–100 cells (exact numbers are provided in the Source Data) from each condition were analyzed. Mitochondrial dysmorphology was more rapidly observed in the order of: ER > Lyso > PM. Representative cells showing Normal, Mix, and Round mitochondria are indicated by gray, green, and magenta arrows in (c), respectively. Similar results have been obtained in three independent experiments. Scale bars, 20 μm.
