Fig. 1: Structure of metazoan FAS, FA synthesis.
From: Engineering metazoan fatty acid synthase to control chain length applied in yeast
a, Crystallographic structure of porcine mFAS (PDB ID: 2VZ8). The homodimeric mFAS adopts an extended X-shaped conformation. Condensing and modifying parts are connected by a short linker and form two lateral reaction clefts. The TE and ACP domains are attached flexibly such that they cannot be traced in electron density. Zoom in shows that one reaction cleft is attached to depict substrate shuttling by ACP. Numbers indicate the sequence of reactions (the path of ACP in shuttling substrates and intermediates to the catalytic domains). b, Cycle of metazoan FA biosynthesis. At defined length, TE releases the acyl chain as free FA. Numbers indicate the sequence of reactions as shown in a.
