Fig. 2: Design of a generalist mFAS.
From: Engineering metazoan fatty acid synthase to control chain length applied in yeast
a, Scheme and domain borders of the TE/‘TesA swap. A cartoon of mFAS is shown for clarity with one protomer colored. b, Exemplary activity assay of mFAS and the mFAS/‘TesA hybrid, monitored by NADPH consumption. The specific activities of WT mFAS and mFAS/‘TesA were 460 and 458 nmol min−1 mg−1, respectively. Inset shows product distributions of mFAS and the mFAS/‘TesA hybrid. The bars represent the means of three biological replicates and the error bars show s.d.
