Fig. 4: In vitro analysis of 12 mFAS/‘TesA hybrids engineered in KS and ‘TesA.
From: Engineering metazoan fatty acid synthase to control chain length applied in yeast
a, Normalized activity monitored by NADPH consumption. The assay was performed in 20 µl volume. Components were used in final concentrations of 100 μM Acetyl-CoA, 100 μM Malonyl-CoA, 50 μM NADPH and 20–80 nM enzyme. Data collected in biological triplicates with three technical replicates each, and normalized to mFAS_WT activity. Error bars represent s.d. b, Product distributions collected in three biological replicates with three technical replicates each. Data collected for hybrid constructs with nonmutated KS highlighted by a gray background.
