Extended Data Fig. 1: Formation of cyclic imides from asparagine.
From: PCMT1 generates the C-terminal cyclic imide degron on CRBN substrates
(a) Scheme of protein damage events that occur through Asn residues and result in the C-terminal cyclic imide or isoaspartate post-translational modifications. (b) Chemical scheme and conversion of model peptides incubated in 20 mM NH4OAc (pH 7.4) buffer at 37 °C (n = 3). We detected two distinct hydrolysis products, which were constitutional isomers, but were unable to assign their identities. Data represent mean ± S.D. (c) Scheme of PCMT1 correction of isoaspartate modifications.
