Extended Data Fig. 5: Sample cryo-EM densities and conformational flexibility analysis. | Nature Chemical Biology

Extended Data Fig. 5: Sample cryo-EM densities and conformational flexibility analysis.

From: Allosteric activation of RNF20/RNF40–RAD6A-mediated H2BK120 monoubiquitylation by H2BS112 GlcNAcylation

Extended Data Fig. 5: Sample cryo-EM densities and conformational flexibility analysis.The alternative text for this image may have been generated using AI.

a, Nucleosmal 147-bp DNA density with atomic model fitted. b, Histone octamer density with cartoon model fitted. c, Sample densities of histone H2A, H2B, H3, and H4, and nucleosomal SHL0 DNA with atomic model fitted. d, Cryo-EM density and model of the H2B αC helix (residues 103–125) modified with O-GlcNAc. e–h, RNF20 RING domain (g) and RNF40 RING domain (h) densities p with cartoon model fitted. i, Conformational flexibility analysis of the complex. Cryo-EM maps and models of three distinct states are shown. j, Structural alignment of the three conformational states, highlighting the rotation of the RNF20/RNF40 RING dimer and the movement of ubiquitin.

Back to article page