Extended Data Fig. 7: Chemically synthesized monoglycosylated H2B variants were incorporated into nucleosomes.
a, Amino acid sequence of histone H2B. The ligation sites A58 and A97, and the glycosylation site S112 are indicated by red and light sea green solid circles, respectively. b, Convergent synthetic route of monoglycosylated H2B variants (G-H2B). The chemical structures of O-D-pyranoses studied here are shown at the bottom. c, Size-exclusion chromatograms of H2A/G-H2B histone dimers. d, Representative CBB-stained gel image of purified H2A/G-H2B histone dimers. e, Representative CBB-stained SDS-PAGE (top) and SYBR-Gold-stained native PAGE (bottom) gel images of purified H2B-monoglycosylated NCPs for biochemical assays. Two independent PAGE analyses in d and e were performed with similar results.
