Extended Data Fig. 10: Surface-specific binding and opsonophagocytosis of H. pylori.
From: Uncovering bacterial pseudaminylation with pan-specific antibody tools
(A) In vitro gentamicin protection assays of H. pylori strains in response to pretreatment with 1E8 Pse-specific antibody, isotype control, or PBS (n = 3 independent experiments for each condition). Box and whisker plots indicate minimum, first quartile, median, third quartile, and maximum values. Kruskal–Wallis tests gave p-values < 0.05 (*) and <0.01 (**). (B) Flow cytometry analysis of H. pylori strains either unstained or stained with α-Pse mAb alone, α-mouse-AlexaFluor647 alone, or both α-Pse mAb 1E8 and α-mouse-AlexaFluor647.
