Extended Data Fig. 2: Butterfly plots of synthetic verses native glycopeptide 281ISFTNDSAVSR291 of FlgE.
From: Uncovering bacterial pseudaminylation with pan-specific antibody tools
Butterfly plots comparing HCD fragmentation of synthetic glycopeptides bearing an (A) α- or (B) β-configured Pse residue with the identical authentic glycopeptides derived from tryptic digests of flagellin preparation of H. pylori P12. Spectral angle (SA) analysis comparing matched b- and y-ion intensities to native 281ISFTNDSAVSR291 of FlgE supports highest similarity to α-pseudaminylation compared to β-pseudaminylation.
